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KMID : 0359320030430030449
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Korean Journal of Veterinary Research
2003 Volume.43 No. 3 p.449 ~ p.456
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Expression of VP2 of Aquatic Birnavirus GC-1 Isolated from Rockfish (Sebastes schlegeli), Rearing in Seawater in Korea
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조성준/Joh, Seong-joon
성환우/이윤정/김재홍/강신영/Sung, Haan-woo/Lee, Yun-jeong/Kim, Jae-hong/Kang, Shien-young
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Abstract
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The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.
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